Application of CRISPR/Cas-based Biosensing Platform in Molecular Diagnostics-OAJRC Cellular and Molecular Biology-Pubscie

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Application of CRISPR/Cas-based Biosensing Platform in Molecular Diagnostics

DOI: 10.26855/oajrccmb.2019.12.001
Year, volume (issue): 2019, 1(1)
pp. 1-3
Published in: OAJRC Cellular and Molecular Biology
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F. Apendi, J. Ibara

Molecular diagnostics, CRISPR/Cas, Biosensing platform, Application value

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Abstract

The CRISPR/Cas system is widely used in genome editing. The CRISPR/Cas system can accurately cut and recognize DNA and RNA sequences. In the process of recognizing target sequences, the CRISPR/Cas system can not only recognize the target sequence to start the targeted cleavage activity, and also show non-specific cleavage activity in DNA, RNA molecules and other single strands, add fluorescent group reporter nucleic acid molecules to the reaction system, and observe through fluorescent signals to the location of the target nucleic acid molecule, and with the help of the different cutting preferences of the Cas protein, it can also detect multiple target molecules at the same time.

Keywords: Molecular diagnostics, CRISPR/Cas, Biosensing platform, Application value

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[1] Koo B, Kim D, Kweon J, et al. CRISPR/dCas9-mediated biosensor for detection of tick-borne diseases [J]. Sensors and Actuators B: Chemical, 2018, 273: 316-321.

[2] Geraldi A, Giri-Rachman E A. Synthetic biology-based portable in vitro diagnostic platforms [J]. Alexandria journal of medicine, 2018, 54(4): 423-428.

[3] Koo B, Kim D, Kweon J, et al. CRISPR/dCas9-mediated biosensor for detection of tick-borne diseases [J]. Sensors and Actuators B: Chemical, 2018, 273: 316-321.

[4] Aquino-Jarquin G. CRISPR-Cas14 is now part of the artillery for gene editing and molecular diagnostic [J]. Nanomedicine: Nanotechnology, Biology and Medicine, 2019, 18: 428-431.

[5] Batista A C, Pacheco L G C. Detecting pathogens with Zinc-Finger, TALE and CRISPR-based programmable nucleic acid binding proteins [J]. Journal of microbiological methods, 2018, 152: 98-104.

[6] Liu L, Yang D, Liu G. Signal amplification strategies for paper-based analytical devices [J]. Biosensors and Bioelectronics, 2019, 136: 60-75.

[7] Otlu B. CRISPR-Cas System: A Brief History and Future in the Diagnosis and Treatment of Infectious Diseases [J]. Infectious Diseases and Clinical Microbiology, 2019, 1(1): 2-6.

[8] Jones K A, Zinkus-Boltz J, Dickinson B C. Recent advances in developing and applying biosensors for synthetic biology [J]. Nano Futures, 2019, 3(4): 042002.

[9] Kim C M, Smolke C D. Biomedical applications of RNA-based devices [J]. Current Opinion in Biomedical Engineering, 2017, 4: 106-115.

[10] Choi K R, Jang W D, Yang D, et al. Systems metabolic engineering strategies: integrating systems and synthetic biology with metabolic engineering [J]. Trends in biotechnology, 2019, 37(8): 817-837.

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